anti cd66b pe  (Elabscience Biotechnology)

Journal: Cell Reports Medicine

Article Title: Surface CD52, CD84, and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors

doi: 10.1016/j.xcrm.2023.101380

Figure Lengend Snippet:

Article Snippet: For cell sorting by flow cytometry, mononuclear cells or granulocytes were incubated with neutrophil specific antibodies as described in the flow cytometry section, and sorted using either a FACS Aria II flow cytometer (Becton Dickinson; University of Verona) or a BD FACS Aria III Cell Sorter (BD Bioscience, IMCES, University of Duisburg-Essen)., Isolation of total CD66b + neutrophils by magnetic bead selection was performed by incubating mononuclear cells or granulocytes with fluorescence-conjugated anti-CD66b mAbs, followed by incubation with specific anti-fluorochrome microbeads (Milteny Biotec).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Cell Isolation, Amplification, Multiplexing, Expressing, Software